FastQCFastQC Report
Wed 6 Dec 2017
SRR837479.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837479.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16663789
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1045430.6273663210689957RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCC253.99347E-544.80638523
ACTAGGA253.99347E-544.80638522
TTAACAG950.044.80638522
TATAGAA1000.044.80638522
AACACGC253.99347E-544.80638522
TACCAGA302.2325585E-644.8063823
GGGGGTA600.044.8063822
TGGCGTT253.9943377E-544.80473721
GTTAGCA4050.044.80473721
AATGCAA551.8189894E-1244.80473721
GTTAACA950.044.80473721
GTTTTGA207.1883714E-444.80473721
TTTTAGA253.9943377E-544.80473721
AATGTAA600.044.80473721
GAAATAG351.2555029E-744.80446220
TGTGGCA454.0199666E-1044.80446220
GAGTAAA207.1885885E-444.80446220
GAGCGGA1250.044.80446220
TGTAAGG800.044.80446220
GGTTAAC950.044.80446220
Produced by FastQC (version 0.11.2)