FastQCFastQC Report
Wed 6 Dec 2017
SRR837478.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837478.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22793574
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG858300.3765534970514058RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCC207.1851845E-444.8088423
GGGTAAT454.0199666E-1044.8088422
TATCGAA302.2317527E-644.8088422
GAACGGA407.0867827E-944.8088422
AGACGCC551.8189894E-1244.8088423
ATAGATT407.0867827E-944.8088423
TAACAGA1400.044.8088423
GTAGGGA1250.044.8088422
TTAACAG1400.044.8088422
GGGGATA253.9922234E-544.8088422
TACCAGA253.9922234E-544.8088423
GATAGTT351.2545752E-744.8088422
GATAGTA207.1851845E-444.8088422
GAGACGC551.8189894E-1244.8088422
GATAGAT407.0867827E-944.8088422
TGTCGAA302.2317527E-644.8088422
AACACGC207.1851845E-444.8088422
GAGGTAA207.186772E-444.80683520
GTATGGG750.044.80683521
GTTTGCA207.186772E-444.80683520
Produced by FastQC (version 0.11.2)