FastQCFastQC Report
Wed 6 Dec 2017
SRR837477.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837477.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19187157
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG335820.1750233241954501RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAGA1050.044.8047323
ATAGGAA1350.044.8047323
TTACCAG1050.044.8047322
ACACGCC454.0199666E-1044.80472623
TCGGGAA253.9943658E-544.80472623
TTAACAG4800.044.80472622
GTGGTAC207.1884075E-444.80472623
TATCGAA502.3646862E-1144.80472622
GATAGAT253.9943658E-544.80472622
GGGATAC351.2554665E-744.80472623
AACACGC454.0199666E-1044.80472622
CTGTCGA454.0199666E-1044.8024621
AGATCGG207.1901997E-444.8024621
AGATAGG1250.044.8024621
CATGAAA1150.044.8024621
AGGGTAA407.0958777E-944.8024621
AGTATTG1500.044.8024621
AATGCAA551.8189894E-1244.8024621
GTTAACT207.1901997E-444.8024621
GTTAACA4800.044.8024621
Produced by FastQC (version 0.11.2)