FastQCFastQC Report
Wed 6 Dec 2017
SRR837476.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837476.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10323437
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG555250.5378538174834602RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGCACTCCAG416070.4030343770199789RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATNGAATTCTCGGGTGCCAAGGAACTCC152370.14759619301207536RNA PCR Primer, Index 1 (96% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC103570.10032511459119671RNA PCR Primer, Index 2 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCA302.239649E-644.78550723
AGCGGAA1000.044.78550721
AGATGGT600.044.78550721
GTTAGAA1200.044.78550722
AGATTTG302.239649E-644.78550721
AGTTCGA254.0043662E-544.78550721
AGTTTGA254.0043662E-544.78550721
AGTTAGA1200.044.78550721
AGAATTG850.044.78550321
AGTATTG551.8189894E-1244.78550321
AGGTGGT551.8189894E-1244.78550321
AGAAATG850.044.78550321
AGTGTAA2350.044.78550321
GTTGGTA207.203461E-444.78550322
GGTGGTT207.203461E-444.78550322
TTGGTAT207.203461E-444.78550323
GTTATTG207.203461E-444.78550321
AGAAGGG351.259632E-744.785521
AGAGGGA12000.044.785521
GAGCGGA1000.044.78395520
Produced by FastQC (version 0.11.2)