FastQCFastQC Report
Wed 6 Dec 2017
SRR837475.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837475.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12773942
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG502610.3934650713147124RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGCACTCCAG460080.3601707288165235RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATNGAATTCTCGGGTGCCAAGGAACTCC201420.15768037775652965RNA PCR Primer, Index 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCA1150.044.78579323
TTCGAAT407.1177055E-944.7857923
TGGGGAT254.0044593E-544.78543522
GTGTAAT3650.044.7854322
GGTGGTA407.1195245E-944.7854322
GTTCGAA351.2596684E-744.7854322
ATGGGGT502.3646862E-1144.78525521
AGTCGAA254.004554E-544.78525521
TTTAGTT254.004554E-544.78525521
AGTGAAA11300.044.78525521
ATGGGGA302.239778E-644.7852521
AGAAGGG351.2597047E-744.7852521
AGTGGAT5000.044.7852521
AGAAATG1200.044.7852521
AGTGTAA3650.044.7852521
AGCGGAA1050.044.7852521
AGATTGA1100.044.7852521
AGTTCGA351.2597047E-744.7852521
AGTTAGA1550.044.7852521
GAGAAGG502.3646862E-1144.78471820
Produced by FastQC (version 0.11.2)