FastQCFastQC Report
Wed 6 Dec 2017
SRR837474.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837474.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12085433
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGCACTCCAG464750.3845538674534872RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATNGAATTCTCGGGTGCCAAGGAACTCC247970.2051808983592065RNA PCR Primer, Index 1 (96% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG201790.16696960712950873RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAAT351.2568307E-744.79825623
TATCTCA1350.044.79825623
GTCGAAT253.9977807E-544.79807322
GTTCGAA302.2353597E-644.79807322
TTATCTC1350.044.79806522
AGAAGGG302.2354907E-644.79769521
AGGTGGT302.2354907E-644.79769521
AGGTGAA302.2354907E-644.79769521
AGTGTAA2950.044.79769521
AGATGGT950.044.79769521
AGTTCGA302.2354907E-644.79769521
ATGGGGA253.9979805E-544.7976921
AGGGGGA5000.044.7976921
AGAGTTG551.8189894E-1244.7976921
AGCGGAA1150.044.7976921
AGTCGAA253.9979805E-544.7976921
AGTTAGA1050.044.7976921
GAGCGGA1150.044.79693620
GAGTCGA253.9983795E-544.79693620
GAGTTGA1150.044.79693620
Produced by FastQC (version 0.11.2)