FastQCFastQC Report
Wed 6 Dec 2017
SRR837473.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837473.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10476962
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG622910.5945521230295576RNA PCR Primer, Index 1 (96% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA449100.4286547951591311RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT143640.13710081223927317RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCCG104810.10003854170703302RNA PCR Primer, Index 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAAT407.1067916E-944.79336523
TATCTCA1150.044.7933623
TTATTGT207.197412E-444.79314822
TTATCTC1150.044.79314422
AGAATTG600.044.7929321
AGAAATG351.2579767E-744.7929321
AGTGTAA2050.044.7929321
GTTATTG254.0004517E-544.7929321
AGTTCGA351.2579767E-744.7929321
GTTATCT1200.044.7929321
ATAGGGA207.197585E-444.79292721
AGATGGG1150.044.79292721
TGTTGGG254.0005674E-544.79271320
TTTATTG254.0005674E-544.79271320
GAGTGAT207.197758E-444.7927120
GAGTAGA3550.044.7927120
GAGGGGT207.197758E-444.7927120
TTTAATG407.1086106E-944.7927120
GATAGGG207.197758E-444.7927120
TGTAAGG207.197758E-444.7927120
Produced by FastQC (version 0.11.2)