FastQCFastQC Report
Wed 6 Dec 2017
SRR837472.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837472.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10256784
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG894830.8724274587434034RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGCACTCCAG430290.41951746278365615RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATNGAATTCTCGGGTGCCAAGGAACTCC162970.15888996004985578RNA PCR Primer, Index 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCT207.1902515E-444.8021924
TATCTCA1050.044.8021923
GGCGTTG207.190604E-444.80174622
AGAGTTG253.996154E-544.8010821
AGAAATG900.044.8010821
AGTATTG351.2561759E-744.80107521
AGGTGGT600.044.80107521
TGGGGAA407.0958777E-944.80107521
AGTGTAA2500.044.80107521
AGATGGT800.044.80107521
TGGCGTT207.191134E-444.80107521
GTTATCT1200.044.80107521
GAGGTGA253.996271E-544.80085420
GAGAAGG502.3646862E-1144.80085420
GAGTGGG16650.044.80085420
TGTGGGA650.044.80085420
GAGGGGT502.3646862E-1144.80085420
AGTTATC1150.044.80085420
TGTTGGG407.0976967E-944.8008520
GAGAAAT950.044.8008520
Produced by FastQC (version 0.11.2)