FastQCFastQC Report
Wed 6 Dec 2017
SRR837471.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837471.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10602248
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG899000.8479333816752823RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGCACTCCAG432780.4081964504131576RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATNGAATTCTCGGGTGCCAAGGAACTCC139550.13162302938018428RNA PCR Primer, Index 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCA1600.044.78929523
TTATCTC1600.044.7888622
TTATTGT254.002603E-544.7888622
ATAGGGA207.200979E-444.78864721
AGGTGGT407.1140676E-944.78864721
AGAAATG900.044.78864721
AGTGTAA2550.044.78864721
AGCGGAA750.044.78864721
AGATTGG13700.044.78864721
AGATTGA750.044.78864721
AGTTCGA207.200979E-444.78864721
AGGGGTA254.0027167E-544.78864321
GAGAAAT950.044.78821620
GAAAATG207.201321E-444.78821620
GAGTATG3700.044.78821620
GTGGGGA351.2590317E-744.78821620
GAGAGTT302.2387212E-644.78821620
GAGAGGA5200.044.78821620
GAGATTG14550.044.78821620
GATAGGG254.0029445E-544.78821620
Produced by FastQC (version 0.11.2)