FastQCFastQC Report
Wed 6 Dec 2017
SRR837470.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837470.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21220553
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG2600441.2254346057805374RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCCG258300.12172161583159496RNA PCR Primer, Index 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTGC301.7390994E-646.4618079
TATTTGA9100.046.46180714
TCGCCCA206.010657E-446.46180713
ATTTACA650.046.46180711
TGTTTAC405.144102E-946.46180713
TATCACT900.046.46180712
AACCGAC301.7390994E-646.46180710
TACCCTC650.046.46180712
GGTACAA1700.046.46180710
CAAACGC1700.046.46180711
ACAAAAG452.8194336E-1046.46180711
GATGTTT359.435644E-846.46180711
TCCGCTC650.046.46180711
CATATCA301.7390994E-646.46180710
TACCATA600.046.4618078
GTACAAC1700.046.46180711
AGATAGC1100.046.46180711
CGACGAC301.7390994E-646.46180710
TCATTCC1200.046.46180711
CATGATA800.046.46180712
Produced by FastQC (version 0.11.2)