FastQCFastQC Report
Wed 6 Dec 2017
SRR837469.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837469.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11559642
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG649690.562032976453769RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG253.2099564E-546.494089
TATTTGC253.2099564E-546.494089
ATTTACA253.2099564E-546.4940811
GAAGCAG1500.046.4940814
AATTTAC253.2099564E-546.4940810
ATTAGAC501.4551915E-1146.4940812
ATGGACA253.2099564E-546.4940812
CGTTGCT253.2099564E-546.4940811
CAATTAC501.4551915E-1146.4940810
GCGTTGC253.2099564E-546.4940810
TCCTACC1000.046.494089
TGTGATC253.2099564E-546.494089
GTTAAAA501.4551915E-1146.4940810
GTAGACT501.4551915E-1146.494088
CTACCCT1000.046.4940811
TTAAAAG501.4551915E-1146.4940811
ACCGTCT253.2099564E-546.4940812
TATACTC501.4551915E-1146.4940812
TGATGTT253.2099564E-546.4940814
TGAGACG253.2099564E-546.4940813
Produced by FastQC (version 0.11.2)