FastQCFastQC Report
Wed 6 Dec 2017
SRR837468.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837468.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17467561
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1631270.9338853890362828RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATAGG301.7311322E-646.49271414
TACATAG205.9909583E-446.49271414
TGCATAG600.046.49271414
GGTATAA2350.046.49257710
TGCTATA12850.046.4925779
GTATAAC2350.046.49257711
TATAACT2350.046.49257712
CTGCTAG3250.046.4925738
TTGGCCC950.046.4925739
GTATTCA253.2106425E-546.49257310
CAGTTGC1100.046.4925738
ATCAGCA253.2106425E-546.49257313
TAGAACG550.046.49257310
CCAATTC253.2106425E-546.49257310
GCCATAT550.046.4925738
TCCTACA4100.046.4925739
AAACGCA1000.046.49257312
ATATCAC550.046.49257311
CAGATCC2050.046.4925739
AACGCTT452.8012437E-1046.4925738
Produced by FastQC (version 0.11.2)