FastQCFastQC Report
Wed 6 Dec 2017
SRR837467.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837467.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24100532
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1690870.7015903217406155RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTAG1300.046.428768
ATCCTAC5550.046.428768
TATCACC301.7476523E-646.4287612
TTGGCCC301.7476523E-646.428769
ACCGGTC1150.046.4287610
TCTTGAG301.7476523E-646.4287614
GGTACAA700.046.4287610
GTACAAC700.046.4287611
AGATAGC600.046.4287611
TCATTCC301.7476523E-646.4287611
TTTAACC600.046.4287613
CTATATT4500.046.4287611
GCAACAT700.046.4287612
CATGACT359.488758E-846.4287611
GCTATAT4500.046.4287610
GCTGCTA301.7476523E-646.428769
TGTGCAC700.046.4287611
CCATGAC359.488758E-846.4287610
ATATCAC301.7476523E-646.4287611
GCTTCTT301.7476523E-646.4287611
Produced by FastQC (version 0.11.2)