FastQCFastQC Report
Wed 6 Dec 2017
SRR837466.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837466.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20675581
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1570750.7597126291154769RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACA700.046.42751711
TGATAGT550.046.42751712
TAGAGAG800.046.42751710
CATATCG405.178663E-946.42751710
AATTTAC700.046.42751710
TTCAGCA405.178663E-946.4275179
CAAAAGT206.032564E-446.42751712
AGATAGC3350.046.42751711
CCTAGTC1250.046.4275178
TAGACGC206.032564E-446.4275179
CAGCCCA206.032564E-446.42751713
ATTACTA206.032564E-446.42751712
GGCAATA359.490577E-846.42751713
TTACTCA405.178663E-946.42751713
TTACTAA206.032564E-446.42751713
TAAAACT359.490577E-846.42751712
GCCATTA405.178663E-946.4275179
TGTGCAC1900.046.42751711
TAGTTCG405.178663E-946.4275179
TTATAGT359.490577E-846.4275179
Produced by FastQC (version 0.11.2)