FastQCFastQC Report
Wed 6 Dec 2017
SRR837465.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837465.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20524908
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1284750.6259467764727618RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAA452.8376235E-1046.42746410
TACCCCG1850.046.42746414
GTACAAC452.8376235E-1046.42746411
GCTGCAC3700.046.42746412
TAAGTGC452.8376235E-1046.4274648
CTGCAGG452.8376235E-1046.42746414
CGTGTCC452.8376235E-1046.4274648
CGCACCG452.8376235E-1046.42746414
GCTACCC1850.046.42746412
CTCCTTC452.8376235E-1046.42746414
AGCTACC1850.046.42746411
TGAGCAG49150.046.4274649
CCGCACC452.8376235E-1046.42746413
TGATCGG650.046.427469
ATTTACA301.7479761E-646.4274611
CATGGTT1100.046.4274612
CTGCTAG2950.046.427468
GTTTGAC501.6370905E-1146.4274614
AACCGGT2250.046.427469
TGATAGG700.046.4274614
Produced by FastQC (version 0.11.2)