FastQCFastQC Report
Wed 6 Dec 2017
SRR837464.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837464.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9467615
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1643291.7356958431452907RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCA207.529507E-444.3847541
AAACCGT13950.044.3847541
TCCTATA3200.044.3847541
TGTCTTA254.2225904E-544.3847541
TATCGCA207.529507E-444.3847541
TGCTGGT207.529507E-444.3847541
ACTCTTT207.529507E-444.3847541
CCTTGCA254.2225904E-544.3847541
TAATGCA254.2225904E-544.3847541
TATTGCA480050.044.3847541
TCAAATC207.529507E-444.3847541
AAAAGCT207.529507E-444.3847541
AATGGCG407.707058E-944.3847541
GTCTACG1450.044.3847541
TACCACA207.529507E-444.3847541
TGTAAAC7000.044.3847541
CATTGCA12250.044.384751
CTATACG600.044.384751
TTCAAGT2850.044.384751
CAACCGT551.8189894E-1244.384751
Produced by FastQC (version 0.11.2)