FastQCFastQC Report
Wed 6 Dec 2017
SRR837463.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837463.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17019359
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG2751691.6168000216694414RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG207.5049105E-444.414461
TATTTCA207.5049105E-444.414461
TGTCAAC502.5465852E-1144.414461
TGGAGGC207.5049105E-444.414461
TACCCTG502.5465852E-1144.414461
TCCATAA407.661583E-944.414461
CTCCGTT254.2060878E-544.414461
CAAAACG254.2060878E-544.414461
TGAAGCG254.2060878E-544.414461
ACACCGT900.044.414461
TTATGGT207.5049105E-444.414461
GTCTACG1850.044.414461
AATGGCG1950.044.414461
TAACGGA4150.044.414461
ACCCTAT207.5049105E-444.414461
ATCACAT207.5049105E-444.414461
TACATTC207.5049105E-444.414461
TATTGCG207.5049105E-444.414461
TAACCGT1500.044.414461
TGTCTTA750.044.414461
Produced by FastQC (version 0.11.2)