FastQCFastQC Report
Wed 6 Dec 2017
SRR837462.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837462.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16956146
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1768671.043084908563538RNA PCR Primer, Index 1 (96% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC688840.40624797639746674RNA PCR Primer, Index 5 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACCA700.044.422331
TAACGGA3500.044.422331
TCGTGTA5750.044.422331
TATTACA351.3429599E-744.422331
TATAGCA351.3429599E-744.422331
TACTAGA551.8189894E-1244.422331
ACCAATA351.3429599E-744.422331
CAGAGTC351.3429599E-744.422331
TAATGCA454.3837645E-1044.422331
TATTTCA254.2016916E-544.4223251
TGTCAAC407.650669E-944.4223251
TCGTTTC254.2016916E-544.4223251
TCTTGCA13500.044.4223251
TCCATAA254.2016916E-544.4223251
CAAAACG302.3688353E-644.4223251
TAAAGAA207.4983644E-444.4223251
TTTCGAT207.4983644E-444.4223251
CTATACG1250.044.4223251
GTCTACG3900.044.4223251
TCAGGCT53200.044.4223251
Produced by FastQC (version 0.11.2)