FastQCFastQC Report
Wed 6 Dec 2017
SRR837461.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837461.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19405906
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG2164301.1152790289719017RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCCG237120.12218960557677647RNA PCR Primer, Index 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGCA351.3533281E-744.3789331
TTCACCA551.8189894E-1244.3789331
TGTCTTA351.3533281E-744.3789331
AATGACA4250.044.3789331
TGTCAGT254.226029E-544.378931
TCTTGCA7100.044.378931
CAACCGT1050.044.378931
AATGGCG950.044.378931
TACGGAA454.4201443E-1044.378931
ATCACAT254.226029E-544.378931
TATTGAA207.5345725E-444.378931
TGTTCCT207.5345725E-444.378931
CCCCCTA254.226029E-544.378931
ACGCTGC302.3848406E-644.378931
TATAGCA207.5345725E-444.378931
TACTAGA207.5345725E-444.378931
CTTTCAG254.226029E-544.378931
TCGACTC207.5345725E-444.378931
CAGAGTC254.226029E-544.378931
TATCGCA254.226029E-544.378931
Produced by FastQC (version 0.11.2)