FastQCFastQC Report
Wed 6 Dec 2017
SRR837460.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837460.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20881569
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG2558171.2250851456612288RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACCA407.734343E-944.3671231
TGTCTTA207.544469E-444.3671231
ACCACTG207.544469E-444.3671231
TTCAAGT2500.044.3671231
TACGGAA302.3892226E-644.3671231
CACCGGA302.3892226E-644.3671231
TTCTGTA18550.044.3671231
TGCTGGT302.3892226E-644.3671231
TGTCAAC207.544469E-444.3671231
TCATGTA9700.044.3671231
AATGGCG302.3892226E-644.3671231
TCCGTAC351.3561657E-744.3671231
AAACGGA207.544469E-444.3671231
AAGCTCG700.044.3671231
AATGACA1400.044.3671231
AAACCGT6700.044.367121
ACGCTGC254.232686E-544.367121
TCGATTC454.4383341E-1044.367121
TCAGGCT13200.044.367121
TCGAAAC254.232686E-544.367121
Produced by FastQC (version 0.11.2)