FastQCFastQC Report
Wed 6 Dec 2017
SRR837459.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837459.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26267481
Sequences flagged as poor quality0
Sequence length50
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTA408.096322E-944.138411
AAACTGG207.739002E-444.138411
TCATTTA1500.044.138411
TCCGATT4350.044.138411
TCTTCTT2050.044.138411
TTTCGTT207.739002E-444.138411
TCTTATT11450.044.138411
CAACCGT700.044.138411
TTTCCTT7400.044.138411
TTTCCTA408.096322E-944.138411
TTTCATG800.044.138411
TTCCATT1650.044.138411
AAACCGC254.3638487E-544.138411
CCCTTGA502.7284841E-1144.138411
TTTGATT15500.044.138411
TCCCCTT502.7284841E-1144.138411
TTACCGC254.3638487E-544.138411
TTACATT351.4124089E-744.138411
TTATTGC1100.044.138411
TAAGATT351.4124089E-744.138411