FastQCFastQC Report
Wed 6 Dec 2017
SRR837458.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837458.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9884613
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1075421.0879738033244195RNA PCR Primer, Index 1 (96% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT116040.11739458085005453RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTGA4600.044.85647214
GACAGAG253.9670922E-544.85647214
GTACCAG1750.044.85647214
CCATAGG502.1827873E-1144.85647214
TTAACCT502.1827873E-1144.85647214
TGTAGCT253.9670922E-544.85647214
TCGAGAC4400.044.85647214
ATCGGTG650.044.85647214
TCGGTTT253.9670922E-544.85647214
GAAATAG207.1474817E-444.8564714
GGTACAG207.1474817E-444.8564714
TACCCCG2800.044.8564714
GAAGCAG1650.044.8564714
TAGACGT407.01948E-944.8564714
ACGCACT407.01948E-944.8564714
TGTATAT800.044.8564714
TGTTGCC302.2153617E-644.8564714
TGCATAG3650.044.8564714
CTAACAG302.2153617E-644.8564714
TATTCAC207.1474817E-444.8564714
Produced by FastQC (version 0.11.2)