FastQCFastQC Report
Wed 6 Dec 2017
SRR837456.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837456.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12215406
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG543680.44507730647675564RNA PCR Primer, Index 1 (96% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT494600.4048985355050827RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCATAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC185220.15162819803124022RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC1800.044.87972314
TCTTGAG406.9885573E-944.87972314
GTACCAC453.965397E-1044.87972313
GAAGCAG4950.044.87972314
TACCACG453.965397E-1044.87972314
TAGACTC253.9550276E-544.87972312
CCAGGTA207.1293267E-444.87972314
TGGACAG750.044.87972313
TTTGTGC12000.044.87972312
CATGATA1800.044.87972312
TTTAACC1100.044.87972313
ATCAGCA453.965397E-1044.87972313
AGCCACG253.9550276E-544.87972313
ATAGCAG3100.044.87972313
TATATTT4550.044.87972312
TTGTATT406.9885573E-944.87972313
GATTTGA900.044.87972314
TGTATAT253.9550276E-544.87972314
CGTCTAG253.9550276E-544.87972314
TAAAAGG1500.044.87972312
Produced by FastQC (version 0.11.2)