FastQCFastQC Report
Wed 6 Dec 2017
SRR837455.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837455.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12130326
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1487521.2262819647221352RNA PCR Primer, Index 1 (96% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT473850.39063253534983317RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCATAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC155370.1280839443226835RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAACC2100.044.87283313
TAAGTTC950.044.87283312
TGACGAA1900.044.87283312
ATTTTGA1900.044.87283313
CTACCCC3550.044.87283313
TTTTGAC1900.044.87283314
ACAACTC253.9586143E-544.8728313
TATTTGA5250.044.8728314
CATGGTT1950.044.8728312
TGTTTAC351.2404962E-744.8728313
TATCACC253.9586143E-544.8728312
CAGCGGC453.965397E-1044.8728313
CTGTCTA1100.044.8728313
TTGGCCA600.044.8728312
TCCATAG1100.044.8728313
GTACCAG5700.044.8728314
AAATCGG253.9586143E-544.8728313
AATTTGC302.209852E-644.8728314
TACCACG253.9586143E-544.8728314
TAGACTC253.9586143E-544.8728312
Produced by FastQC (version 0.11.2)