FastQCFastQC Report
Wed 6 Dec 2017
SRR837451.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837451.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20369705
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC7701883.781046411815979RNA PCR Primer, Index 5 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACCA254.2099527E-544.4076041
TTCACAG254.2099527E-544.4076041
TAGTGCA254.2099527E-544.4076041
TGGTGCA254.2099527E-544.4076041
TCCCTTA254.2099527E-544.4076041
CTTTCAG207.5106556E-444.40761
AAACCGT13550.044.40761
CTATACG302.3742705E-644.40761
TGTCTTA207.5106556E-444.40761
TTATTGC2200.044.40761
TTTCATT10150.044.40761
TTTCAAC207.5106556E-444.40761
TCGTGTA8450.044.40761
TATTGCG207.5106556E-444.40761
CCCATAA4250.044.40761
GTCCTTT207.5106556E-444.40761
TATCCTT454.3837645E-1044.40761
AGACGAT407.672497E-944.40761
CCATTGC207.5106556E-444.40761
TCGTTTC454.3837645E-1044.40761
Produced by FastQC (version 0.11.2)