FastQCFastQC Report
Wed 6 Dec 2017
SRR837449.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837449.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17012813
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT444160.26107381536492524RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGC351.3436147E-744.4195631
TACTAGA351.3436147E-744.4195631
TGTCAGT800.044.419561
TCGCATT254.203237E-544.419561
TTTAAAC1600.044.419561
TTTCGAT407.654307E-944.419561
CTATACG302.3698522E-644.419561
TTATGGT407.654307E-944.419561
GTCTACG2700.044.419561
TAACGGA254.203237E-544.419561
GCTGACT850.044.419561
TCCTATA4950.044.419561
TCGATCC207.5006654E-444.419561
TATTGTA502.5465852E-1144.419561
TATTGCC207.5006654E-444.419561
TATTACA207.5006654E-444.419561
AAACAGT207.5006654E-444.419561
CTTTCAG207.5006654E-444.419561
TGGTGCA207.5006654E-444.419561
TAGCTTA207.5006654E-444.419561
Produced by FastQC (version 0.11.2)