FastQCFastQC Report
Wed 6 Dec 2017
SRR837448.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837448.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18884147
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT268660.14226747970136008RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAAC900.044.428141
TTCAATT454.3655746E-1044.428141
TCTCATT5950.044.428141
TTAGGTA9100.044.4281351
CCCCTAA1750.044.4281351
TAAAGTG1000.044.4281351
CAACGTA502.5465852E-1144.4281351
ACCATCG254.1984676E-544.4281351
TTTAATT13050.044.4281351
TTATAAA254.1984676E-544.4281351
ACGATGC351.3415774E-744.4281351
TATTCCA351.3415774E-744.4281351
TCTTGCA2400.044.428131
TTGTGTA207.493562E-444.428131
CAAAACG207.493562E-444.428131
TTTACAC207.493562E-444.428131
ACACCGT3200.044.428131
TAACGGA207.493562E-444.428131
TATTGTA407.6415745E-944.428131
TAACCGT407.6415745E-944.428131
Produced by FastQC (version 0.11.2)