FastQCFastQC Report
Wed 6 Dec 2017
SRR837447.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837447.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18772312
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT463100.2466931084460987RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC1450.044.411521
TCGAAAC1450.044.411521
TTAGGTA2300.044.4115141
CAACGGG254.207751E-544.4115141
CTATACA254.207751E-544.4115141
GTCTACG2400.044.4115141
TTCAAAT254.207751E-544.4115141
TATTGTA502.5465852E-1144.4115141
AATCTGC6750.044.4115141
TACTAGA650.044.4115141
CTTTCAG600.044.4115141
TTTTGCA650.044.4115141
TGCAGTA302.3728207E-644.4115141
AGAGGTA1150.044.4115141
TGTACAC2000.044.4115141
TATCGCA254.207751E-544.4115141
TCTTGCA3250.044.411511
TATTGCG207.507382E-444.411511
TATTCAT207.507382E-444.411511
TCTCATT5650.044.411511
Produced by FastQC (version 0.11.2)