FastQCFastQC Report
Wed 6 Dec 2017
SRR837446.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837446.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13399846
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG543890.40589272443877344RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTTC207.759001E-444.11512425
GTTTTCG207.759001E-444.11512426
CTCAATT207.759001E-444.11512426
ATCCTTC207.759001E-444.11512424
TGGAGTA254.378023E-544.11396822
TTAGTTG207.7600026E-444.11396822
TTTTCGC408.138159E-944.11264827
TTCACAG254.4381537E-544.0121461
CTATACG254.4381537E-544.0121461
CAACCGT3050.044.0121461
TATCGCA254.4381537E-544.0121461
ACATTGA254.4381537E-544.0121461
AATGGCG502.7284841E-1144.0121461
TCTTGCA1650.044.0121461
CATGCCT254.4381537E-544.0121461
TTCACCA207.848817E-444.0121421
CACATTC351.4444959E-744.0121421
ACGCTGC207.848817E-444.0121421
TCCTATA5850.044.0121421
AGAGGTA351.4444959E-744.0121421
Produced by FastQC (version 0.11.2)