FastQCFastQC Report
Wed 6 Dec 2017
SRR837445.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837445.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17707055
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG729570.4120222137447475RNA PCR Primer, Index 1 (96% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA223660.12631123583227138RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT187780.1060481260153086RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTTA254.3735927E-544.12163524
TTGTTAA207.7534345E-444.1216325
AATCTCT207.7534345E-444.1216326
CTTTGAA207.7534345E-444.1216325
GCTTTGA207.7534345E-444.1216324
CTCAATT551.8189894E-1244.1216326
TTATTGT351.4169746E-744.12013222
TCAATTG408.127245E-944.11938527
TTTTCGC302.4830624E-644.1193827
AGAACAT302.4830624E-644.1193827
ATTTCGC302.4830624E-644.1193827
TTTCGCG600.044.1193828
TCCTTTA1600.044.011141
TTCACGT254.4388093E-544.011141
CCAACGG207.849767E-444.011141
TCCTATA7600.044.011141
TGTCTTA351.4447869E-744.011141
CAACCGT3900.044.011141
AGAGGTA1000.044.011141
AGCTACA302.5253903E-644.011141
Produced by FastQC (version 0.11.2)