FastQCFastQC Report
Wed 6 Dec 2017
SRR837444.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837444.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13286127
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG760470.5723789935170723RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCA1250.044.11713423
GTTCGCC207.7574025E-444.1169726
TCACGTT207.758124E-444.11613522
GTTGGTA351.4179568E-744.11613522
TTAGTTG207.758124E-444.11613522
GGGGGTA750.044.1161322
TTCGCCT207.7595667E-444.11446827
TTATTGC1850.044.0121041
CTTTCAG302.5249628E-644.01211
TCCTTTA1200.044.01211
TTTTGCA207.8488514E-444.01211
TTCACAG551.8189894E-1244.01211
CCAACGG207.8488514E-444.01211
TCCTATA6400.044.01211
GATGCCT207.8488514E-444.01211
TCGAAAC551.8189894E-1244.01211
TATCGCA302.5249628E-644.01211
ACACCGT207.8488514E-444.01211
TGTCAGT302.5249628E-644.01211
TATTGTA700.044.01211
Produced by FastQC (version 0.11.2)