FastQCFastQC Report
Wed 6 Dec 2017
SRR837443.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837443.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20930552
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG957020.4572359104528156RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGTT351.4170655E-744.1198323
GGAGTAT254.3746746E-544.1198323
TATCTCA1050.044.11982723
ATTGTTA254.3747365E-544.11972824
TGGTACT351.4171019E-744.11972424
TCTCAAT1050.044.11972425
GCCGATC207.755209E-444.1196226
TTATCTC1100.044.11898422
GTTGGTA800.044.11898422
GGGGATA207.7557587E-444.11898422
AGAACAT207.757498E-444.11697827
GAACATG207.757498E-444.11697828
TTTTGCA254.4384447E-544.0118031
TTCACGT254.4384447E-544.0118031
CTATACG502.7284841E-1144.0118031
TTATTGC2000.044.0118031
TATCGCA254.4384447E-544.0118031
CCCATCA254.4384447E-544.0118031
TATTGCG254.4384447E-544.0118031
CAGAGTC254.4384447E-544.0118031
Produced by FastQC (version 0.11.2)