FastQCFastQC Report
Wed 6 Dec 2017
SRR837442.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837442.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16174535
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1287670.7961094399313489RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTCT302.5010631E-644.07305526
GATATTC454.7293724E-1044.0697825
ATTGTTA207.80916E-444.05749524
AAGTATT254.4112967E-544.0574924
TGGTACT254.4112967E-544.0574924
GGATATT302.5071495E-644.0574924
GGTGGTA850.044.0558622
TGGAGTA254.4122648E-544.05585522
GGAGTAT207.8107085E-444.05571723
TAGCCGC650.044.0246351
TAGCAGC6750.044.0246351
TGAAGCG302.5200552E-644.0246351
AATGACA2200.044.0246351
TTATGGT502.7284841E-1144.024631
CTTTCAG207.837907E-444.024631
TTCACGT207.837907E-444.024631
TTCACAG207.837907E-444.024631
AACCGGG254.4307693E-544.024631
TCGACTC207.837907E-444.024631
TGTCTTA254.4307693E-544.024631
Produced by FastQC (version 0.11.2)