FastQCFastQC Report
Wed 6 Dec 2017
SRR837441.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837441.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17065783
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG979460.5739320604275818RNA PCR Primer, Index 1 (96% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA248920.14585911469752075RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTCG302.5015033E-644.0719526
TGGGATA302.5078298E-644.05578222
GTTGTAA302.5078298E-644.05578222
AGGGTAT207.810782E-444.0556523
TATCTCA700.044.05564523
AGGGTGA207.834747E-444.0282531
CAGCTCG254.4302404E-544.025541
TTCACCA600.044.025541
CCAACGG254.4302404E-544.025541
CTATACG551.8189894E-1244.025541
CAACCGT2450.044.025541
AGAGGTA551.8189894E-1244.025541
TATCGCA302.519706E-644.025541
TATTGCT254.4302404E-544.025541
GGATATC254.4302404E-544.025541
TCATGTA6700.044.025541
TCCACTC254.4302404E-544.025541
ATCACAT254.4302404E-544.025541
CACTAGA850.044.025541
ACTGGAC302.519706E-644.025541
Produced by FastQC (version 0.11.2)