FastQCFastQC Report
Wed 6 Dec 2017
SRR837440.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837440.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16709685
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG857760.5133310412494311RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCTC207.796575E-444.07193427
GTTTTCG502.7284841E-1144.07193426
AGTTTTC302.502591E-644.06915725
TGGTACT254.4118264E-544.05660624
TATGAAA351.4336183E-744.05462622
TGGGGTC254.428717E-544.028131
TTTTGTA207.8368426E-444.0258561
CAACCGT2250.044.0258561
TAACGGA207.8368426E-444.0258561
TAGCCGC1050.044.0258561
TATTGTA800.044.0258561
TATTGAA850.044.0258561
GATTGCA207.8368426E-444.0258561
TCAGTGC351.4409852E-744.0258561
CACCCCG700.044.0258561
TCTTGCA800.044.0258561
ATCACAT207.8368426E-444.0258561
TCCGTAC351.4409852E-744.0258561
ACTGGAC207.8368426E-444.0258561
ATATTGC351.4409852E-744.0258561
Produced by FastQC (version 0.11.2)