FastQCFastQC Report
Wed 6 Dec 2017
SRR837439.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837439.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15161461
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG672430.4435126667542132RNA PCR Primer, Index 1 (96% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA207310.13673484369349365RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCG207.7974366E-444.0709226
AAATCCT254.405332E-544.0675725
AGTCTTC207.800356E-444.0675725
TCTCAAT502.7284841E-1144.0675725
AAGTCTT408.232746E-944.0546124
TTATCTC502.7284841E-1144.053322
TGGAGTA408.236384E-944.053322
GGGGGTT650.044.053322
GGGGGTA1100.044.053322
GTAGGGA254.413761E-544.053322
GTTGGAC254.413761E-544.053322
GGGGATA207.812805E-444.053322
GTTGTAA408.236384E-944.053322
TATCTCA502.7284841E-1144.05315423
TAGGGAA207.8129326E-444.05315423
CACATTC254.4291915E-544.0272671
TATCGCA254.4291915E-544.0272671
TAGCCGC1000.044.0272671
CGAATGT254.4291915E-544.0272671
TCTTGTA7950.044.0272671
Produced by FastQC (version 0.11.2)