FastQCFastQC Report
Wed 6 Dec 2017
SRR837438.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837438.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17394349
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG413010.2374391821159849RNA PCR Primer, Index 1 (96% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT399210.2295055710334431RNA PCR Primer, Index 1 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA227020.13051365130135079RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAA454.7293724E-1044.07401328
TACTCGC750.044.07401327
GTACTCG600.044.0740126
TTTTCGC254.40156E-544.0740127
GTCTTCG207.794776E-444.0740126
GAGTACT351.4327816E-744.05788824
AAGTCTT207.808831E-444.05788824
GGAGTTT351.4333091E-744.05586223
GGAGTAC351.4333091E-744.05586223
CTTTCAG254.4292545E-544.0272061
TCCTTTA1500.044.0272061
TTCACGT254.4292545E-544.0272061
TGTCTTA1000.044.0272061
TAGCTTA254.4292545E-544.0272061
TATCGCA351.4406578E-744.0272061
TCGTGTA4250.044.0272061
TATTGAA900.044.0272061
TAGCAGC10850.044.0272061
GCTGACT1000.044.0272061
TTATGGT408.278221E-944.0272031
Produced by FastQC (version 0.11.2)