FastQCFastQC Report
Wed 6 Dec 2017
SRR837437.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837437.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17830678
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1211670.6795423034390503RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCAAT551.8189894E-1244.06921425
GTAATCG207.7989604E-444.0692125
ATCTCAA551.8189894E-1244.0568324
GCTTTGA207.8097597E-444.0568324
TTATCTC551.8189894E-1244.05534722
TATCTCA551.8189894E-1244.05534723
GGTGGTA1200.044.05534722
CTTTCAG207.8376767E-444.024921
CGCTCGG950.044.024921
TATCGCA207.8376767E-444.024921
TAGCCGC551.8189894E-1244.024921
ACCAATA800.044.024921
CCCATCA207.8376767E-444.024921
TATTGAA551.8189894E-1244.024921
TGAAGCG207.8376767E-444.024921
GTCTACG1350.044.024921
TCCGTAC207.8376767E-444.024921
ATTCCCA207.8376767E-444.024921
TCTCACA207.8376767E-444.024921
TCCTTTA1750.044.0249181
Produced by FastQC (version 0.11.2)